Chloroplast Genome Sequencing: Some Reflections

Multiplexed Fragaria Chloroplast Genome Sequencing

A method to sequence multiple chloroplast genomes using ultra high throughput sequencing technologies was recently described. Complete chloroplast genome sequences can resolve phylogenetic relationships at low taxonomic levels and identify informative point mutations and indels. The objective of this research was to sequence multiple Fragaria chloroplast genomes using the Illumina Genome Analyz...

Exploring the History of Chloroplast Capture in Arabis Using Whole Chloroplast Genome Sequencing

Chloroplast capture occurs when the chloroplast of one plant species is introgressed into another plant species. The phylogenies of nuclear and chloroplast markers from East Asian Arabis species are incongruent, which indicates hybrid origin and shows chloroplast capture. In the present study, the complete chloroplast genomes of A. hirsuta, A. nipponica, and A. flagellosa were sequenced in orde...

Sequencing of Chloroplast Genome Using Whole Cellular DNA and Solexa Sequencing Technology

Sequencing of the chloroplast (cp) genome using traditional sequencing methods has been difficult because of its size (>120 kb) and the complicated procedures required to prepare templates. To explore the feasibility of sequencing the cp genome using DNA extracted from whole cells and Solexa sequencing technology, we sequenced whole cellular DNA isolated from leaves of three Brassicarapa access...

Shallow Whole Genome Sequencing for the Assembly of Complete Chloroplast Genome Sequence of Arachis hypogaea L.

Professional education: Some reflections

It was a courageous decision to found a journal in the early 1980’s; and it was nothing less than a leap of faith to name a journal: Education for Information. The times were not auspicious: people were becoming less certain as to what ‘Education’ was; and it was often said of ‘Information’ that it told one more about the user of the term than it did about its intended objective correlative. Li...